17P Increased expression of POU5F1 in metastatic colorectal cancer is not caused by increased expression of OCT4A and OCT4B isoforms

The gene POU5F1 (also known as an OCT4) belonging to POU protein family is composed of 5 exons that can create many isoforms by alternative splicing. Most isoform OCT4A expressed in types stem cells. Its nuclear localization and presence the DNA-binding domain enable it regulate expression genes are responsible for pluripotency blocking differentiation Other splicing products numerous OCT4B (OCT4B-OCT4B5) differ from cell absence domain. Majority OCT4 were discovered only recently there have not been clear evidences about their role cells yet. OCT4A, OCT4B1 tumorigenesis already explored but without definite results. aim our research analysis variants association with patient´s prognosis occurrence metastasis. For this we used fresh surgical specimens patients colorectal cancer isolated primary tumours (n=47) metastasis (n=31). Subsequently, total RNA was transcribed cDNA. Through quantitative PCR method using particular TaqMan assays specific ΔΔ Ct method, separately analyzed relative A, B, also all together. We found out tumour significantly lower than adjacent non-tumour tissue. On other hand, increased metastatic tumor compared observed experiment probe whereas neither B reason Our indicate play a pivotal exclude A cause metastasis, consider important identify transcript.